Fig. 3. Etanercept hampers neutrophil accumulation at injured serosa. Mice were administered phosphate-buffered saline (PBS) (A) or etanercept (100 mg/moues) (B) and the cecal lesions were sampled at the indicated time points after cecum cauterization for hematoxylin and eosin staining or immunohistochemistry for Ly6G. (C) We counted total Ly6G⁺ cells in the serosa or adhesion indicated by the green dotted line (A, B), quantified the Ly6G-positive proportion to the area, and calculated the relative ratio of each proportion to that of tissue slices of the control group at 6 h. Data are given as means ± SEM. *p<0.05 indicated statistically significant differences. ns, not significant. Three mice were used for each experimental group. We performed the experiments three times with similar results.